In Situ Hybridization for BEGINNERS
In situ hybridization is a technique used by scientists to label DNA and RNA, but why do we need to label DNA/RNA? Here I explain the purpose of In Situ Hybridizations, provide a brief description of the two major types (chromogen and fluorescence in situ hybridization (FISH)), give several examples of their use, and explain some drawbacks. This is a part of my Slice of Science series where I "briefly" describe some interesting scientific techniques. Items used in the video: Music: https://www.youtube.com/watch?v=wJcEKFrqICY&list=PLZLeGaOtm7T_UDvZrLNPhNw5ktXBXVRnj&index=2&t=608s Articles used as examples: Cancer Cell DNA Translocation Example Louzada S, Adega F, Chaves R. Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2. PLoS One. 2012;7(1):e29923. doi: 10.1371/journal.pone.0029923. Epub 2012 Jan 10. PMID: 22253826; PMCID: PMC3254647. Figure 2A Dentate Gyrus/Low Level Chromogen RNA In situ Caramello A, Galichet C, Rizzoti K, Lovell-Badge R. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife. 2021 Jan 4;10:e63904. doi: 10.7554/eLife.63904. PMID: 33393905; PMCID: PMC7806271. Figure supplement 5A Enthesis Example/Double RNA FISH Kult S, Olender T, Osterwalder M, Markman S, Leshkowitz D, Krief S, Blecher-Gonen R, Ben-Moshe S, Farack L, Keren-Shaul H, Salame TM, Capellini TD, Itzkovitz S, Amit I, Visel A, Zelzer E. Bi-fated tendon-to-bone attachment cells are regulated by shared enhancers and KLF transcription factors. Elife. 2021 Jan 15;10:e55361. doi: 10.7554/eLife.55361. PMID: 33448926; PMCID: PMC7810463. Figure 1A, Figure 2C These articles are distributed under the terms of the Creative Commons Attribution License (CC-BY-4.0), which permits unrestricted use and redistribution provided that the original author and source are credited. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/
In situ hybridization is a technique used by scientists to label DNA and RNA, but why do we need to label DNA/RNA? Here I explain the purpose of In Situ Hybridizations, provide a brief description of the two major types (chromogen and fluorescence in situ hybridization (FISH)), give several examples of their use, and explain some drawbacks. This is a part of my Slice of Science series where I "briefly" describe some interesting scientific techniques. Items used in the video: Music: https://www.youtube.com/watch?v=wJcEKFrqICY&list=PLZLeGaOtm7T_UDvZrLNPhNw5ktXBXVRnj&index=2&t=608s Articles used as examples: Cancer Cell DNA Translocation Example Louzada S, Adega F, Chaves R. Defining the sister rat mammary tumor cell lines HH-16 cl.2/1 and HH-16.cl.4 as an in vitro cell model for Erbb2. PLoS One. 2012;7(1):e29923. doi: 10.1371/journal.pone.0029923. Epub 2012 Jan 10. PMID: 22253826; PMCID: PMC3254647. Figure 2A Dentate Gyrus/Low Level Chromogen RNA In situ Caramello A, Galichet C, Rizzoti K, Lovell-Badge R. Dentate gyrus development requires a cortical hem-derived astrocytic scaffold. Elife. 2021 Jan 4;10:e63904. doi: 10.7554/eLife.63904. PMID: 33393905; PMCID: PMC7806271. Figure supplement 5A Enthesis Example/Double RNA FISH Kult S, Olender T, Osterwalder M, Markman S, Leshkowitz D, Krief S, Blecher-Gonen R, Ben-Moshe S, Farack L, Keren-Shaul H, Salame TM, Capellini TD, Itzkovitz S, Amit I, Visel A, Zelzer E. Bi-fated tendon-to-bone attachment cells are regulated by shared enhancers and KLF transcription factors. Elife. 2021 Jan 15;10:e55361. doi: 10.7554/eLife.55361. PMID: 33448926; PMCID: PMC7810463. Figure 1A, Figure 2C These articles are distributed under the terms of the Creative Commons Attribution License (CC-BY-4.0), which permits unrestricted use and redistribution provided that the original author and source are credited. https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/